Definition of Gram stain of tissue biopsy:

Gram stain of tissue biopsy test involves using crystal violet stain to test a sample of tissue taken from a biopsy.

The Gram stain method can be used on almost any specimen. It is an excellent technique for making a general, basic identification of the type of bacteria in the sample.

How the test is performed:

A sample, called a smear, from a tissue specimen is placed in a very thin layer on a microscope slide. The specimen is stained with crystal violet stain and goes through more processing before it is examined under the microscope for bacteria.

Characteristics of the bacteria, such as their color, shape, clustering (if any), and pattern of staining help determine the type of bacteria.

How to prepare for the test:

If the biopsy is included as part of a surgical procedure, you will be asked not to eat or drink anything the night before surgery. If the biopsy is of a superficial (on the surface of the body) tissue, you may be asked not to eat or drink for several hours before the procedure.

How the test will feel:

How the test feels depends on the part of the body being biopsied. There are several different methods for taking tissue samples.

• A needle may be inserted through the skin to the tissue.
• A cut (incision) through the skin into the tissue may be made, and a small piece of the tissue removed.
• A biopsy may also be taken from inside the body using an instrument that helps the doctor see inside the body, such as an endoscope or cystoscope.

You may feel pressure and mild pain during a biopsy. Some form of pain relieving medicine (anesthetic) is usually given so you have little or no pain.

Why the test is performed:

The test is performed when an infection of a body tissue is suspected.