Definition of 5'-nucleotidase :

5'-nucleotidase (5'-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood.

How the test is performed:

Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.

Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.

Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.

How to prepare for the test:

Your health care provider will instruct you, if necessary, to discontinue drugs that may interfere with the test. Drugs that may interfere with results include:

• Acetaminophen (Tylenol)
• Halothane
• Isoniazid
• Methyldopa
• Nitrofurantoin

How the test will feel:

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed:

Your doctor may order this test if you have signs of a liver problem. It is used mostly to tell if the high protein level is due to liver damage or skeletal muscle damage.