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Anticuerpo fluorescente directo en esputo - Overview

Nombres alternativos

Prueba de inmunofluorescencia directa; Anticuerpos fluorescentes directos en esputo; AFD

Definición:

Es un examen para buscar microorganismos en las secreciones pulmonares.

Forma en que se realiza el examen:

Usted producirá una muestra de esputo de los pulmones, expectorando moco desde lo profundo de estos órganos. (El moco no es lo mismo que saliva o escupitajo de la boca).

En el laboratorio, los anticuerpos, que han sido químicamente ligados a un tinte fluorescente, se añaden a la muestra. Estos anticuerpos se consideran "marcados". Ellos se fijarán a antígenos específicos, en este caso, el microorganismo contra el cual se formaron. Si el microorganismo específico está presente, se puede observar un resplandor brillante (fluorescencia) en la muestra de esputo, empleando un microscopio especial.

Preparación para el examen:

Si no se obtiene una muestra de esputo por medio de la expectoración, se puede administrar un tratamiento respiratorio antes del examen para desencadenar la producción de dicho esputo.

Lo que se siente durante el examen:

No se presenta ninguna molestia.

Razones por las que se realiza el examen:

El médico puede ordenar este examen si uno presenta signos de ciertas infecciones pulmonares o neumonía.

  • Reviewed last on: 5/23/2010
  • David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.
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